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KMID : 0043320070300040475
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Archives of Pharmacal Research
2007 Volume.30 No. 4 p.475 ~ p.480
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Antithrombotic Effects by Oral Administration of Novel Proteinase Fraction from Earthworm Eisenia andrei on Venous Thrombosis Model in Rats
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Lee Chul-Kyu
Shin Jang-Sik
Kim Byung-Su
Cho Il-Hwan
Kim Yeong-Shik
Lee Eun-Bang
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Abstract
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A novel proteinase fraction, SPP-501, was purified from the earthworm, Eisenia andrei, and its antithrombotic effects compared with those of urokinase and t-PA (tissue type-plasminogen activator) in a thrombosis model, induced by the insertion of a stainless wire coil into the inferior vena cava. SPP-501, urokinase and t-PA were administrated once a day for 14 days. On the oral administration of SPP-501, as well as urokinase and t-PA, the thrombus weight was dramatically decreased. The euglobulin lysis time (ELT) was also shortened by SPP-501, but urokinase and t-PA failed to dissolve the euglobulin clot. Conversely, urokinase and t-PA produced detectable fibrinogen/fibrin degradation products (FDP), but SPP-501 did not. Thrombin induced platelet aggregation was desensitized in the SPP-501 treatment groups. With a high dose of SPP-501 (45 mg/kg), the APTT (activated partial thromboplastin time) was prolonged. These results suggest that SPP-501 shows both antithrombotic and fibrinolytic activities when orally administered.
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KEYWORD
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Antithrombosis, Fibrinolysis, Lumbrokinases, Venous thrombosis model
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